Hand-tighten the PEEK fittings, then turn them an extra 1/4 turn with a wrench if necessary. Do not overtighten. 3. Front Panel Controls and Interface
Inject a standard sample to verify retention times and peak shapes before starting a sequence. Shutdown Sequence
Regular maintenance extends the life of your detector and ensures data integrity.
The Shimadzu SPD-20A is a UV-Vis detector designed for use in HPLC systems. Its advanced design and high-sensitivity photodiode array (PDA) enable accurate detection of analytes in a wide range of applications. The instrument features:
Loosen the retaining screws holding the lamp assembly and carefully pull the old lamp straight out without twisting. shimadzu spd20a user manual
Perform a wavelength calibration/calibration check as described in the service manual. Conclusion
Shimadzu SPD-20A Service Manual | PDF | Optical Filter | Light
Press the LAMP key on the keypad (or activate it via LabSolutions software). Allow the Deuterium ( D2cap D sub 2
The deuterium lamp has a limited lifespan (approx. 2000 hours). Hand-tighten the PEEK fittings, then turn them an
Allow the detector to warm up for at least 30-60 minutes to ensure baseline stability.
The SPD-20A/AV includes a solvent recycling valve option ( SV.WASTE ). This component can be programmed to divert the initial flow of solvent (which may contain impurities) to waste and then switch to send the rest to a collection vessel for recycling. This feature helps reduce solvent consumption and operating costs.
The detector achieves a noise level as low as ±0.25 × 10⁻⁵ AU and a linearity of 2.5 AU , ensuring high-quality data for a variety of applications.
Refer to the for detailed procedures.
To ensure accurate and reliable results, it's crucial to follow proper operating procedures when using the Shimadzu SPD-20A. Here's a step-by-step guide to get you started:
Ensure the unit is placed in a vibration-free environment to maintain detector stability. 3. Key Operating Procedures
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Available on the SPD-20AV variant, which allows for simultaneous detection using deuterium and tungsten lamps (190 to 900 nm). 2. Key Components Explained in the Manual Front Panel Controls and Interface Inject a standard
Another vital configuration is the setting. While standard absorbance is measured as positive peaks (upward), the SPD-20A allows for negative polarity, which is useful when monitoring peaks that have lower absorbance than the mobile phase (vacancy peaks) or for specific refractive index applications.
The display should indicate "D2" when the lamp is operational. C. Flow Cell Maintenance and Cleaning
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